Catalog number: SBB-CE0107, 25 μg

Parkin (Parkinson juvenile disease protein 2) is a ~52 kDa E3 Ligase enzyme protein, encoded by PARK2 gene. It plays an important role in the ubiquitin-proteasome system and acts as a regulator of protein breakdown. This protein has been recombinantly expressed in insect cells and in vivo biotinylated.

Catalog number: SBB-UP0172, 25 μg

The array of cellular processes initiated and regulated by ubiquitin has been partially explained by the structural diversity of differently linked ubiquitin chains. In a ubiquitin chain, ubiquitin moieties can be conjugated through one of their lysine residues (K6, K11, K27, K29, K33, K48 and K63) or the N-terminal methionine residue (M1), offering countless possibilities to assemble a specific polymer. Ubiquitin molecules can also be modified by other post-translational modifications, including acetylation and phosphorylation, adding another layer of ubiquitin signal regulation and diversification.

Catalog number: SBB-PS0002, 50 μg

This product consists of a full-length, mature ISG15 polypeptide (amino acids 2-157) recombinantly expressed in E.coli, conjugated on its c-terminus to a quenched Rhodamine110 dye. Hydrolysis of the conjugate results in fluorescence observable by excitation at 485nm and emission at 535nm, which substantially reduces the risk of autofluorescence of compounds in screenings (Hassapien et al., 2007). Typical working range is 50-500nM.

Catalog number: SBB-KF0035, 400 wellsThe kit uses ubiquitin labeled with either Europium-Cryptate or Cyanine5 as FRET pair donor and acceptor fluorophores respectively, completely eliminating the need for antibody based detection setups.

Enzymatic incorporation of the labeled ubiquitins into chains conjugated onto ITCH leads to an increase in fluorescence emission at 665 nm (Emacceptor) and decrease at emission wavelength 620 nm (Emdonor).

Catalog number: SBB-CE0058, 50 μg

UBA1 is the canonical Ubiquitin E1 activating enzyme, which catalyzes the first step of the Ubiquitin conjugation cascade. UBA1 charges Ubiquitin to a thioester on its active site cysteine through an adenylate intermediate.[1][2][3][4]

Catalog number: SBB-PS0001, 50 μg

This product consists of a full-length, mature ubiquitin polypeptide (amino acids 1-76) recombinantly expressed in E.coli, conjugated on its c-terminus to a quenched Rhodamine110 dye. Hydrolysis of the conjugate results in fluorescence observable by excitation at 485nm and emission at 535nm. Typical working range is 50-500nM.