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Catalog number: SBB-SC0116, 25 μg
Cereblon (CRBN) is the substrate recognition component of DCX (DDB1-CUL4-X-box) E3 Ubiquitin ligase complexes that mediates the ubiquitination of numerous target proteins such as ILF2 and MEIS2. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. In vivo, the endogenous substrate of CRBN MEIS2 is blocked from binding by immunomodulatory drugs (IMiDs), i.e. Thalidomide, which instead promotes the recruitment of IKZF1 or IKZF3 for degradation. DDB1 and CRBN complex has been co-expressed and purified from insect cells Sf9.
$295.00
Catalog number: SBB-SC0116, 25 µg
Cereblon (CRBN) is the substrate recognition component of DCX (DDB1-CUL4-X-box) E3 Ubiquitin ligase complexes that mediates the ubiquitination of numerous target proteins such as ILF2 and MEIS2. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1.
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$295.00
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| Quantity: | 25 µg |
|---|---|
| Molecular Weight: | DDB1 (127 kDa), CRBN (51 kDa) |
| Purity: | >98% by SDS-PAGE |
| Storage Buffer: | 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP |
| Storage | Store at −80°C after product arrival. Avoid multiple freeze/thaws. |
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Open Certificate in New TabCitations and References
1) Wang H., et al. “Histone H3 and H4 ubiquitylation by the CUL4-DDB-ROC1 ubiquitin ligase facilitates cellular response to DNA damage” DOI: 10.1016/j. molcel.2006.03.035 2) Ito T., et al. “Identification of a primary target of thalidomide teratogenicity” DOI: 10.1126/science.1177319 3) Fischer E.S., et al. “Structure of the DDB1-CRBN E3 ubiquitin ligase in complex with thalidomide” DOI: 10.1038/ nature13527 4) Baek K., et al. “NEDD8 nucleates a multivalent cullin- RING-UBE2D ubiquitin ligation assembly” DOI: 10.1038/ s41586-020-2000-y 5) J Wade Harper and Brenda A Schulman, Cullin-RING Ubiquitin Ligase Regulatory Circuits: A Quarter Century Beyond the F-Box Hypothesis” DOI: 10.1146/annurev-biochem-090120-013613
