Catalog number: SBB-TR0015, 50 μg
Highly purified recombinant protein, which has been labeled with a single Cyanine5 (Cy5) moiety at a specific site that keeps all lysines within the protein available and has a fully functional C-terminus that allows for further conjugation into ubiquitin chains and does not affect or impairs the E1 – E2 and E3 conjugation cascade. Cy5-Ubiquitin has an excitation maximum Ex646nm and an emission maximum Em662nm. This protein was expressed in E.coli.[1][2][3][4]
Cy5-Ubiquitin, human recombinant
Ubiquitin is a highly conserved protein that plays a major role in the ubiquitylation pathway, which is conserved from yeast to mammals. Ubiquitylation, the conjugation of ubiquitin to other proteins through a covalent bond between its C-terminal glycine and the ε-amino group of lysine residues or the α-amino group of an N- terminal methionine) onto proteins is essential for many cellular process primarily linked to protein degradation. This process involves three steps with specific groups of enzymes in an ATP depended manner, which are activation with ubiquitin-activating enzymes (E1s), conjugation with ubiquitin-conjugating enzymes (E2s), and ligation with ubiquitin ligases (E3s).