Catalog No. SBB-DE0129, 50 µg
The human SARS-CoV-2 coronavirus harbors two proteases Papain Like Protease PLpro and 3CLpro (Chymotrypsin like Protease) or Mpro (Main-Protease) which is a C30-type cysteine protease. The viral genome encodes more than 20 proteins, with 3CLpro located within the non-structural protein 5 (nsp5) section of the viral polypeptide that cleaves together with PLpro polyproteins (PP1A and PP1AB) into individual functional components.
His10-SARS-CoV-2 Mpro (3CLpro)
The human SARS-CoV-2 coronavirus harbors two proteases Papain Like Protease PLpro and 3CLpro (Chymotrypsin like Protease) or Mpro (Main-Protease) which is a C30-type cysteine protease. The viral genome encodes more than 20 proteins, with 3CLpro located within the non-structural protein 5 (nsp5) section of the viral polypeptide that cleaves together with PLpro polyproteins (PP1A and PP1AB) into individual functional components.
3CLpro recognizes the peptide sequence LQ[S/A/G] where it cleaves c-terminal to the amino acid glutamine (use product SBB-PS0130, KTSAVLQ-Rh110-Glu as universal substrate). The protease 3CLpro is a potential drug target for coronavirus infections due to its essential role in processing the polyproteins that are translated from the viral RNA. The X-ray structures of the unliganded SARS-CoV-2 protease 3CLpro and its complex with an α-ketoamide inhibitor provides a basis for design of α-ketoamide inhibitors. This SARS Coronavirus recombinant 3CLpro is N-terminally His10-tagged and expressed in E.coli.