Neurodegenerative Disease Tools
Catalog number: SBB-CE0055, 50 μg
Parkin (Parkinson juvenile disease protein 2) is a ~52 kDa E3 Ligase enzyme protein, encoded by PARK2 gene. It plays an important role in the ubiquitin-proteasome system and acts as a regulator of protein breakdown. This protein has been recombinantly expressed in insect cells and is untagged.
Catalog number: SBB-CE0107, 25 μg
Parkin (Parkinson juvenile disease protein 2) is a ~52 kDa E3 Ligase enzyme protein, encoded by PARK2 gene. It plays an important role in the ubiquitin-proteasome system and acts as a regulator of protein breakdown. This protein has been recombinantly expressed in insect cells and in vivo biotinylated.
Catalog number: SBB-KF0036, 400 wells
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The kit uses ubiquitin labeled with either Europium-Cryptate or Cyanine5 as FRET pair donor and acceptor fluorophores respectively, completely eliminating the need for antibody based detection setups. Enzymatic incorporation of the labeled ubiquitins into chains conjugated onto Parkin leads to an increase in fluorescence emission at 665 nm (Emacceptor) and decrease at emission wavelength 620 nm (Emdonor).